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cd271 microbeads kits  (Miltenyi Biotec)
96
Miltenyi Biotec cd271 microbeads kits
Immunofluorescence characterisation of oesophageal CSCs indicating the expression of CD44, CD90, and <t>CD271</t> oesophageal CSC surface markers. The micrographs represent three independent assays (50 μm scale bar and magnification: 200x).
Cd271 Microbeads Kits, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd271 microbeads kits/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
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flexable mouse igg2a labeling kit  (Proteintech)
96
Proteintech flexable mouse igg2a labeling kit
(A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control <t>IgG</t> values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
Flexable Mouse Igg2a Labeling Kit, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
flexable mouse igg2a labeling kit - by Bioz Stars, 2026-04
96/100 stars
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anti sca 1 microbead kit  (Miltenyi Biotec)
93
Miltenyi Biotec anti sca 1 microbead kit
(A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control <t>IgG</t> values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
Anti Sca 1 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
anti sca 1 microbead kit - by Bioz Stars, 2026-04
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cd271 microbead kit  (Miltenyi Biotec)
96
Miltenyi Biotec cd271 microbead kit
(A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control <t>IgG</t> values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
Cd271 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd271 microbead kit/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
cd271 microbead kit - by Bioz Stars, 2026-04
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rat anti-mouse cd117 (c-kit) fitc  (Thermo Fisher)
90
Thermo Fisher rat anti-mouse cd117 (c-kit) fitc
(A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control <t>IgG</t> values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
Rat Anti Mouse Cd117 (C Kit) Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-mouse cd117 (c-kit) fitc/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
rat anti-mouse cd117 (c-kit) fitc - by Bioz Stars, 2026-04
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human cd271 microbeads kit  (Miltenyi Biotec)
96
Miltenyi Biotec human cd271 microbeads kit
(A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control <t>IgG</t> values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.
Human Cd271 Microbeads Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cd271 microbeads kit/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
human cd271 microbeads kit - by Bioz Stars, 2026-04
96/100 stars
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Image Search Results


Immunofluorescence characterisation of oesophageal CSCs indicating the expression of CD44, CD90, and CD271 oesophageal CSC surface markers. The micrographs represent three independent assays (50 μm scale bar and magnification: 200x).

Journal: Frontiers in Immunology

Article Title: Cell death mechanisms induced by gold nano-immunoconjugates-mediated photodynamic therapy against human oesophageal cancer stem cells

doi: 10.3389/fimmu.2025.1585251

Figure Lengend Snippet: Immunofluorescence characterisation of oesophageal CSCs indicating the expression of CD44, CD90, and CD271 oesophageal CSC surface markers. The micrographs represent three independent assays (50 μm scale bar and magnification: 200x).

Article Snippet: The CSCs were isolated from the HKESC-1 cells utilising the CD44, CD90, and CD271 Microbeads Kits (130-095-194/130-096-253/130-099-023, Miltenyi Biotec) via a magnetic separation method.

Techniques: Immunofluorescence, Expressing

Nano-immuno-conjugate characterization. TEM analysis of AuNPs (A) and NIC (B) . (C) Spectra analysis of AuNPs, Anti-CD271 antibody, AlPcS 4 Cl, and NIC showing Anti-CD271 antibody at 278nm, a red shift of AuNP from 520 nm to 542 nm in the NIC and AlPcS 4 Cl at 350 nm and 672 nm.

Journal: Frontiers in Immunology

Article Title: Cell death mechanisms induced by gold nano-immunoconjugates-mediated photodynamic therapy against human oesophageal cancer stem cells

doi: 10.3389/fimmu.2025.1585251

Figure Lengend Snippet: Nano-immuno-conjugate characterization. TEM analysis of AuNPs (A) and NIC (B) . (C) Spectra analysis of AuNPs, Anti-CD271 antibody, AlPcS 4 Cl, and NIC showing Anti-CD271 antibody at 278nm, a red shift of AuNP from 520 nm to 542 nm in the NIC and AlPcS 4 Cl at 350 nm and 672 nm.

Article Snippet: The CSCs were isolated from the HKESC-1 cells utilising the CD44, CD90, and CD271 Microbeads Kits (130-095-194/130-096-253/130-099-023, Miltenyi Biotec) via a magnetic separation method.

Techniques:

(A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control IgG values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.

Journal: PLOS Pathogens

Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion

doi: 10.1371/journal.ppat.1013755

Figure Lengend Snippet: (A) A schematic diagram of the RNA immunoprecipitation (RIP) assay used to detect METTL3-associated N mRNA. (B) 293T cells were transfected with METTL3 expression plasmids. At 24 h post-transfection (hpt), the cells were infected with rBPIV3-EGFP at an MOI of 1 for 48 h. The infected cells were lysed, and the extracts were subjected to RNA immunoprecipitation assay with anti-FLAG antibody (Ab). After immunoprecipitation, cDNA of BPIV3-N mRNA captured by METTL3 was synthesized and the amount of N mRNA was quantified by qPCR. Followed by normalization to input RNA values, relative enrichment values of N mRNA were expressed relative to control IgG values using the ΔΔCt method. (C) SRAMP was used to predict the m6A site of the N gene derived from the BPIV3 genome. The vertical axis shows the m6A score and the horizontal axis shows the position number of the base of the N gene where N6-methyladenosine is present within the m6A motif. A yellow bar indicates low-scoring m6A site, N327, and red bars indicate high-scoring m6A sites, N872, N1146, N1372, N1427, and N1443. (D) Schematic representation of the MeRIP (m6A RNA immunoprecipitation) assay. (i) The m6A-containing RNA fragments were bound by an m6A-specific Ab. (ii) Viral N mRNA containing m6A modifications was fragmented. (iii) Antibody–RNA complexes were captured using protein affinity beads. (iv) RNA was eluted, reverse transcribed into cDNA, and amplified by qPCR using primers specific for the indicated N gene regions (N327, N872, N1146, and N1372–1443). (E) 293T cells were infected with rBPIV3-EGFP at an MOI of 1. At 72 h post-infection, the infected cells were harvested, and only mRNA from total RNA was purified. The m6A-modified mRNA was immunoprecipitated with m6A-specific Ab and magnetic beads. Followed by cleavage of the captured mRNA with the cleavage enzyme, the m6A-specific Ab-captured mRNA was eluted from magnetic beads and purified. cDNA was synthesized by reverse transcription reaction, and the m6A sites in N mRNA were then identified by qPCR. The relative value of each to the mRNA value of input RNA was calculated using the ΔΔCt method. Data represent the mean ± SD from n = 3 independent experiments. Asterisks represent statistically significant differences (* p < 0.05). ns; not significant.

Article Snippet: For dsRNA detection, mouse anti-dsRNA Ab was directly labeled using a FlexAble mouse IgG2a labeling kit (Proteintech).

Techniques: RNA Immunoprecipitation, Transfection, Expressing, Infection, Immunoprecipitation, Synthesized, Control, Derivative Assay, Reverse Transcription, Amplification, Purification, Modification, Magnetic Beads